Coding

Part:BBa_K4739006

Designed by: Huaiyuan Ma   Group: iGEM23_SUSTech-Shenzhen   (2023-10-10)


VgrG3-KLA fusion protein

This part is a fusion protein of VgrG3 from V. cholerae, KLA, and 3 v5 tag for western blot. VgrG3 proteins puncture the target cell membrane to deliver the toxic effector molecules. Various VgrG proteins might be associated with different effectors and might target different cells, helping the bacteria to deal with various competitive environments and hosts. In T6SS formation of V. cholerae, VgrG3 will form a trimer, assemble to the tip of T6SS and puncture target cell. Testing results can be viewed in BBa_K4739003.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1978
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1947
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1978
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1978
    Illegal NgoMIV site found at 2035
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None